Anticorps Recombinant de lapin anti-CTSA

CTSA Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251086D9

N° de cat : 80272-3-RR

Synonymes

cathepsin A, EC:3.4.16.5, GLB2, GSL, Lysosomal protective protein

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Galerie de données de validation

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  WB analysis using 80272-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80272-3-RR (CTSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 80272-3-RR

  Biolayer interferometry (BLl) kinetic assays of 80272-3-RR against Human CTSA were performed. The affinity constant is 0.717 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules DU 145, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80272-3-RR cible CTSA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CTSA Protéine recombinante Ag7073
• Nom complet: cathepsin A
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54-60, 32, 20 kDa
• Numéro d’acquisition GenBank: BC000597
• Symbole du gène: Cathepsin A
• Identification du gène (NCBI): 5476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).

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