Anticorps Polyclonal de lapin anti-Cathepsin B

• Phare
• Validé par KD/KO

Cathepsin B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(87)

Avis (1)

N° de cat : 12216-1-AP

Synonymes

CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain

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Galerie de données de validation

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  WB analysis using 12216-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12216-1-AP (Cathepsin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12216-1-AP

  WB result of Cathepsin B antibody (12216-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin B transfected HepG2 cells.

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  IHC staining of human ovary tumor using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A375 using 12216-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Cathepsin B antibody (12216-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 12216-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Cathepsin B (12216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HepG2, cellules HT-1080
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A375,
• Résultats positifs en FC (Intra): cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 68 publications below
• IHC: See 8 publications below
• IF: See 21 publications below

Informations sur le produit

12216-1-AP cible Cathepsin B dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, bovin, Humain, porc, souris, Grenouille
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin B Protéine recombinante Ag2898
• Nom complet: cathepsin B
• Masse moléculaire calculée: 339 aa, 38 kDa
• Poids moléculaire observé: 25 kDa, 31 kDa, 43 kDa
• Numéro d’acquisition GenBank: BC010240
• Symbole du gène: Cathepsin B
• Identification du gène (NCBI): 1508
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

Publications

Species	Application	Title
mouse	WB,IF	Nature; Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.; Authors - Jiasheng Zhang
rat	WB	Cell Death Differ; NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injury; Authors - Jialing Peng
human	WB	Autophagy; Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α; Authors - Qinyu Li
human	IF	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
mouse	WB,IHC	Aging Cell; Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy; Authors - Jian Sun
human	WB	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Weihao (Verified Customer) (02-03-2022)

Works well for mouse BMDM and human macrophages, recommend it

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500

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