Anticorps Polyclonal de lapin anti-Cathepsin D

• Phare
• Validé par KD/KO

Cathepsin D Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

97

Avis (2)

N° de cat : 21327-1-AP

Synonymes

cathepsin D, CLN10, CPSD, CTSD

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Galerie de données de validation

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  WB analysis using 21327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 21327-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21327-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HepG2, cellules HuH-7, cellules MCF-7
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 86 publications below
• IHC: See 13 publications below
• IF: See 12 publications below

Informations sur le produit

21327-1-AP cible Cathepsin D dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin D Protéine recombinante Ag15254
• Nom complet: cathepsin D
• Masse moléculaire calculée: 412 aa, 45 kDa
• Poids moléculaire observé: 32 kDa, 48 kDa, 52 kDa
• Numéro d’acquisition GenBank: BC016320
• Symbole du gène: CTSD
• Identification du gène (NCBI): 1509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)

Publications

Species	Application	Title
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
mouse	WB	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB	Acta Pharm Sin B; Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.; Authors - Xiliang Du
mouse	WB,IF	Redox Biol; Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury; Authors - Yu Xu
human,mouse	WB,IF	Autophagy; Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective.; Authors - M Iqbal Hossain ; KD Validated
mouse	WB	Cell Death Differ; Programmed cell death 4 modulates lysosomal function by inhibiting TFEB translation.; Authors - Xiaotong Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chen (Verified Customer) (06-01-2021)

Very good to detect cathepsin D in postmortem human samples with Western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: postmortem human brain lysate

Cathepsin D Antibody Western Blot validation (1:3000 dilution) in postmortem human brain lysate (Cat no:21327-1-AP)

FH

Efil (Verified Customer) (05-29-2019)

cells were fixed in cold MeOH for 8 minutes

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human fibroblasts

Cathepsin D Antibody Immunofluorescence, validation (1:100 dilution) in human fibroblasts (Cat no:21327-1-AP)

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