Anticorps Polyclonal de lapin anti-Cathepsin K

• Phare
• Validé par KD/KO

Cathepsin K Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(95)

Avis (2)

N° de cat : 11239-1-AP

Synonymes

CTSK, Cathepsin X, catK, CTSO, CTSO2

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Galerie de données de validation

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  WB analysis using 11239-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11239-1-AP (Cathepsin K antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of ROS1728 using 11239-1-AP

  IP result of anti-Cathepsin K (IP:11239-1-AP, 3ug; Detection:11239-1-AP 1:500) with ROS1728 cells lysate 3200ug.

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  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11239-1-AP (Cathepsin K antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human osteosarcoma using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:100.

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  IHC staining of human osteosarcoma using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:100.

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  IF Staining of mouse brain using 11239-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 11239-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of ROS1728 using 11239-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using Cathepsin K antibody (11239-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MG U-87, cellules ROS1728
• Résultats positifs en IP: cellules ROS1728
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain, tissu d'ostéosarcome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules ROS1728,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 68 publications below
• IHC: See 14 publications below
• IF: See 19 publications below

Informations sur le produit

11239-1-AP cible Cathepsin K dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin K Protéine recombinante Ag1761
• Nom complet: cathepsin K
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 27 kDa, 38~46 kDa
• Numéro d’acquisition GenBank: BC016058
• Symbole du gène: Cathepsin K
• Identification du gène (NCBI): 1513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSK(cathepsin K), also named CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts (PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot (PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Circadian control of the secretory pathway maintains collagen homeostasis.; Authors - Joan Chang
mouse	IHC,IF	Biomaterials; BMP2 immune complexes promote new bone formation by facilitating the direct contact between osteoclasts and osteoblasts.; Authors - Yamei Xu
mouse	WB,IF	Dev Cell; Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption; Authors - Mobai Li
mouse	WB	ACS Appl Mater Interfaces; Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis; Authors - Wenzheng Liao
mouse	WB	Br J Pharmacol; Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis.; Authors - Tongling Huang
mouse	IHC	Arthritis Rheum; Osteoarthritic change is delayed in a Ctsk-knockout mouse model of osteoarthritis.; Authors - Kozawa Eiji E

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isabel (Verified Customer) (07-03-2020)

Antibody worked really well to detect Cathepsin K in protein from mouse osteoclasts. Blot was really clean with no other bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary mouse osteoclasts

Cathepsin K Antibody Western Blot validation (1:500 dilution) in Primary mouse osteoclasts (Cat no:11239-1-AP)

FH

RICHA (Verified Customer) (10-09-2019)

Good but hard to get the signal

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from mouse tail tendon

Cathepsin K Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:11239-1-AP)

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