Anticorps Recombinant de lapin anti-CUEDC2

CUEDC2 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251583E6

N° de cat : 86610-2-PBS

Synonymes

C10orf66, CUE domain containing 2, CUE domain-containing protein 2, HOYS6

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Galerie de données de validation

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  WB analysis using 86610-2-RR (same clone as 86610-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86610-2-RR (CUEDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86610-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 86610-2-RR (same clone as 86610-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86610-2-RR (CUEDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86610-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02553-1

  The mean CUEDC2 concentration was determined to be 51.3 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load, 49.9 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02553-1

  Cytometric bead array standard curve of MP02553-1, CUEDC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86610-1-PBS. Detection antibody: 86610-2-PBS. Standard: Ag13847. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 86610-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86610-2-RR against Human CUEDC2 were performed. The affinity constant is 0.925 nM.

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Informations sur le produit

86610-2-PBS cible CUEDC2 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CUEDC2 Protéine recombinante Ag13847
• Nom complet: CUE domain containing 2
• Masse moléculaire calculée: 287 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000262
• Symbole du gène: CUEDC2
• Identification du gène (NCBI): 79004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif-containing protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrine resistance in breast cancer.

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