Anticorps Polyclonal de lapin anti-CUGBP2

CUGBP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12921-1-AP

Synonymes

Bruno like protein 3, BRUNOL3, CELF 2, CELF2, CUG BP2, CUGBP2, ETR 3, ETR3, hNAPOR, NAPOR, RNA binding protein BRUNOL 3

---

Galerie de données de validation

• 

  WB analysis using 12921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12921-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12921-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12921-1-AP

  IP Result of anti-CUGBP2 (IP:12921-1-AP, 3ug; Detection:12921-1-AP 1:300) with mouse skeletal muscle tissue lysate 7500ug.

• 

  IHC staining of human breast cancer using 12921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12921-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12921-1-AP (CUGBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules MCF-7, tissu de thymus de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12921-1-AP cible CUGBP2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CUGBP2 Protéine recombinante Ag3594
• Nom complet: CUG triplet repeat, RNA binding protein 2
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC036391
• Symbole du gène: CUGBP2
• Identification du gène (NCBI): 10659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The CELF (CUGBP and ETR-3 Like Factor)/Bruno-like protein family plays important roles in the regulation of alternative splicing and translation. CUGBP2 is a member of this family of proteins that possesses several alternatively spliced exons. CUGBP2 induces smooth muscle-specific exon inclusion via binding to uridine purine repeat elements (URE) in ACTN1. Although CUGBP2 is known as a splicing activator for the N-methyl-D-aspartate receptor 1 (NMDA R1) exon 21, CUGBP2 represses NMDA R1 exon 5 inclusion. In addition, CUGBP2 was reported to repress the inclusion of the Ir exon 11.

Publications

Species	Application	Title
human	WB	Oncotarget; Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells.; Authors - Bo Li
mouse	IHC	Brain Res; Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition.; Authors - Otsuka Noboru N
human	WB	Exp Biol Med (Maywood); Hypoxic colorectal cancer-secreted exosomes deliver miR-210-3p to normoxic tumor cells to elicit a protumoral effect.; Authors - Liuqing Ge
human	WB,IF	Pharmacol Res; Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway; Authors - Ying Zhang