Anticorps Recombinant de lapin anti-CUL1

CUL1 Recombinant Antibody for Indirect ELISA, IP, WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

Indirect ELISA, IP, WB

Conjugaison

Non conjugué

CloneNo.

5K17

N° de cat : 82474-1-PBS

Synonymes

Cullin-1, Cullin1, CUL-1, 5K17

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Galerie de données de validation

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  WB analysis using 82474-1-RR (same clone as 82474-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82474-1-RR (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 82474-1-RR (same clone as 82474-1-PBS)

  WB result of CUL1 antibody (82474-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL1 transfected A549 cells. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82474-1-RR (same clone as 82474-1-PBS)

  IP result of anti-CUL1 (IP:82474-1-RR, 4ug; Detection:82474-1-RR 1:1000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82474-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82474-1-RR against Human CUL1 were performed. The affinity constant is 21.7 nM.

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Informations sur le produit

82474-1-PBS cible CUL1 dans les applications de Indirect ELISA, IP, WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CUL1 Protéine recombinante Ag3585
• Nom complet: cullin 1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC034318
• Symbole du gène: CUL1
• Identification du gène (NCBI): 8454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

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