Anticorps Polyclonal de lapin anti-CUL3

• Phare
• Validé par KD/KO

CUL3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 11107-1-AP

Synonymes

CUL 3, CUL3, cullin 3, KIAA0617

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Galerie de données de validation

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  WB analysis using 11107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11107-1-AP (CUL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11107-1-AP (CUL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11107-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11107-1-AP (CUL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11107-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11107-1-AP (CUL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 11107-1-AP

  Immunofluorescent analysis of HEK-293 cells using 11107-1-AP (CUL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules PC-12, cellules SH-SY5Y, tissu cérébral de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

11107-1-AP cible CUL3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CUL3 Protéine recombinante Ag1555
• Nom complet: cullin 3
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 80-89 kDa
• Numéro d’acquisition GenBank: BC039598
• Symbole du gène: CUL3
• Identification du gène (NCBI): 8452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination; Authors - Xin Wang
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
human	WB	Food Chem; Tangeretin maintains antioxidant activity by reducing CUL3 mediated NRF2 ubiquitination.; Authors - Yue Wang
human	WB	Aging (Albany NY); Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.; Authors - Xiao Dong ; KD Validated
human	WB,IF,IP	FEBS J; CUL3 induces mitochondrial dysfunction via MRPL12 ubiquitination in renal tubular epithelial cells; Authors - Xingzhao Ji
human	WB	Sci Rep; H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression.; Authors - Xing-Hui Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brittney (Verified Customer) (11-26-2018)

10ug load of protein. Cells lysed in RIPA buffer then added to 2X Laemmli buffer for denaturing. Heated at 95deg for 10 minutes as well.Multiple bands, but all follow same pattern. Assumed the band at ~75 kD was the correct one.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HK-2 Cells

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