Anticorps Polyclonal de lapin anti-CUL3

CUL3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28087-1-PBS

Synonymes

CUL-3, Cullin-3, KIAA0617

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Galerie de données de validation

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  WB analysis using 28087-1-AP (same clone as 28087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28087-1-AP (CUL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

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  WB analysis using 28087-1-AP (same clone as 28087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28087-1-AP (CUL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 28087-1-AP (same clone as 28087-1-PBS)

  IP result of anti-CUL3 (IP:28087-1-AP, 4ug; Detection:28087-1-AP 1:600) with mouse testis tissue lysate 1680 ug. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 28087-1-AP (same clone as 28087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28087-1-AP (CUL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 28087-1-AP (same clone as 28087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28087-1-AP (CUL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28087-1-PBS cible CUL3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CUL3 Protéine recombinante Ag28004
• Nom complet: cullin 3
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 80-88 kDa
• Numéro d’acquisition GenBank: BC039598
• Symbole du gène: CUL3
• Identification du gène (NCBI): 8452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

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