Anticorps Recombinant de lapin anti-CUL3

CUL3 Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240534C7

N° de cat : 83619-5-PBS

Synonymes

240534C7, CUL-3, Cullin-3, KIAA0617

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Galerie de données de validation

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  WB analysis using 83619-5-RR (same clone as 83619-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83619-5-RR (CUL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83619-5-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 83619-5-RR (same clone as 83619-5-PBS)

  IP result of anti-CUL3 (IP:83619-5-RR, 4ug; Detection:83619-5-RR 1:4000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 83619-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83619-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83619-5-RR against Human CUL3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83619-5-PBS cible CUL3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CUL3 Protéine recombinante Ag1555
• Nom complet: cullin 3
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 80-89 kDa
• Numéro d’acquisition GenBank: BC039598
• Symbole du gène: CUL3
• Identification du gène (NCBI): 8452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

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