Anticorps Monoclonal anti-CUL4A

• Phare
• Validé par KD/KO

CUL4A Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, singe, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1A7F12

N° de cat : 66038-1-Ig

Synonymes

CUL 4A, CUL4A, cullin 4A

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Galerie de données de validation

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  WB analysis using 66038-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of pig brain using 66038-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66038-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 66038-1-Ig

  IP Result of anti-CUL4A (IP:66038-1-Ig, 4ug; Detection:66038-1-Ig 1:500) with MCF-7 cells lysate 2800ug.

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  IHC staining of human heart using 66038-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 66038-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 66038-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 66038-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 66038-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 66038-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 66038-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66038-1-Ig (CUL4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, tissu cérébral de porc
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66038-1-Ig cible CUL4A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, singe, souris

• Réactivité: Humain, porc, rat, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CUL4A Protéine recombinante Ag18089
• Nom complet: cullin 4A
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 77 kDa, 88 kDa
• Numéro d’acquisition GenBank: BC008308
• Symbole du gène: CUL4A
• Identification du gène (NCBI): 8451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Publications

Species	Application	Title
human		Curr Biol; Temporal Regulation of ESCO2 Degradation by the MCM Complex, the CUL4-DDB1-VPRBP Complex, and the Anaphase-Promoting Complex.; Authors - Masashi Minamino
human	WB	Oncogene; GSK3β-driven SOX2 overexpression is a targetable vulnerability in esophageal squamous cell carcinoma; Authors - Li Kang ; KD Validated
mouse	WB,IF,IP	Stem Cell Res Ther; KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA.; Authors - Wan Wang