Anticorps Monoclonal anti-CUL4A

• Phare
• Validé par KD/KO

CUL4A Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, singe, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A7F12

N° de cat : 66038-1-PBS

Synonymes

1A7F12, CUL 4A, CUL-4A, cullin 4A

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Galerie de données de validation

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  WB analysis using 66038-1-Ig (same clone as 66038-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66038-1-Ig (same clone as 66038-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66038-1-Ig (same clone as 66038-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 66038-1-Ig (same clone as 66038-1-PBS)

  IP result of anti-CUL4A (IP:66038-1-Ig, 4ug; Detection:66038-1-Ig 1:500) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66038-1-Ig (same clone as 66038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66038-1-Ig (same clone as 66038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66038-1-Ig (same clone as 66038-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66038-1-Ig (CUL4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66038-1-PBS cible CUL4A dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, singe, souris

• Réactivité: Humain, porc, rat, singe, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CUL4A Protéine recombinante Ag18089
• Nom complet: cullin 4A
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 77 kDa, 88 kDa
• Numéro d’acquisition GenBank: BC008308
• Symbole du gène: CUL4A
• Identification du gène (NCBI): 8451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

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