Anticorps Monoclonal anti-CUL4B

• Phare
• Validé par KD/KO

CUL4B Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4E10B10

N° de cat : 60151-1-PBS

Synonymes

4E10B10, cullin 4B, Cullin-4B, KIAA0695

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Galerie de données de validation

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  WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

  various lysates were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

  WB result of CUL4B antibody (60151-1-Ig, 1:500) with si-Control and si-CUL4B transfected HeLa cells. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60151-1-Ig (same clone as 60151-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60151-1-Ig (same clone as 60151-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60151-1-PBS cible CUL4B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CUL4B Protéine recombinante Ag3563
• Nom complet: cullin 4B
• Masse moléculaire calculée: 913 aa, 104 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC036216
• Symbole du gène: CUL4B
• Identification du gène (NCBI): 8450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

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