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Anticorps Monoclonal anti-Cullin 5

Cullin 5 Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

ELISA

Conjugaison

Non conjugué

CloneNo.

4A3E9

N° de cat : 60093-1-Ig

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of Y79 cells using Cullin 5 Monoclonal antibody (60093-1-Ig)

    WB analysis of Y79 using 60093-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 60093-1-Ig (Cullin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using Cullin 5 Monoclonal antibody (60093-1-Ig)

    IHC staining of human skin using 60093-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin slide using 60093-1-Ig (Cullin 5 antibody at dilution of 1:50 (under 40x lens).

Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

60093-1-Ig cible Cullin 5 dans les applications de ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène Cullin 5 Protéine recombinante Ag5453
Nom complet cullin 5
Masse moléculaire calculée 91 kDa
Numéro d’acquisition GenBankBC063306
Symbole du gène CUL5
Identification du gène (NCBI) 8065
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocole

Product Specific Protocols
WB protocol for Cullin 5 antibody 60093-1-IgDownload protocol
IHC protocol for Cullin 5 antibody 60093-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols