Anticorps Polyclonal de lapin anti-CUL7

• Phare
• Validé par KD/KO

CUL7 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13738-1-AP

Synonymes

CUL 7, CUL7, cullin 7, KIAA0076

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Galerie de données de validation

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  WB analysis of HEK-293 using 13738-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13738-1-AP (CUL7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13738-1-AP

  WB result of CUL7 antibody (13738-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HEK-293 cells.

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Applications testées

• Résultats positifs en WB: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below

Informations sur le produit

13738-1-AP cible CUL7 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CUL7 Protéine recombinante Ag4675
• Nom complet: cullin 7
• Masse moléculaire calculée: 1698 aa, 191 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC033647
• Symbole du gène: CUL7
• Identification du gène (NCBI): 9820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. CUL7 was originally discovered as 185-kDa protein associated with the large T antigen of simian virus 40 (SV40). CUL7-deficient mice exhibit neonatal lethality with reduced size and vascular defects. CUL7 presumably plays a role in the DNA damage response by limiting p53 activity. CUL7 mutations have also been identified in 3-Msyndrome and the Yakuts short stature syndrome, both of which are characterized by pre- and post-natal growth retardation but with relatively normal mental and endocrine functions, suggesting that CUL7 may also be crucial for human placental development.

Publications

Species	Application	Title
	WB	Diabetes; Liver kinase b1 is required for white adipose tissue growth and differentiation.; Authors - Zhang Wencheng W
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated
rat	WB	Am J Physiol Renal Physiol; Ubiquitination of NKCC2 by the Cullin-RING E3 Ubiquitin Ligase Family in the Rat Thick Ascending Limb of the Loop of Henle; Authors - Gustavo R Ares
human	WB	Adv Sci (Weinh); Targeting Methylglyoxal Metabolism to Enhance Ferroptosis Sensitivity in Tumor Therapy; Authors - Xinyue Zhang ; KO Validated

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