Anticorps Monoclonal anti-CUL7

• Phare
• Validé par KD/KO

CUL7 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC

Conjugaison

Non conjugué

CloneNo.

2E3G9

N° de cat : 67034-1-Ig

Synonymes

CUL 7, CUL7, cullin 7, dJ20C7.5, KIAA0076

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 67034-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67034-1-Ig

  WB result of CUL7 antibody (67034-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 67034-1-Ig

  WB result of CUL7 antibody (67034-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HeLa cells.

• 

  WB analysis of NCI-H1299 using 67034-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67034-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 67034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules NCI-H1299
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67034-1-Ig cible CUL7 dans les applications de WB, IHC et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CUL7 Protéine recombinante Ag6943
• Nom complet: cullin 7
• Masse moléculaire calculée: 1698 aa, 191 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC033647
• Symbole du gène: CUL7
• Identification du gène (NCBI): 9820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

he cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. CUL7 was originally discovered as 185-kDa protein associated with the large T antigen of simian virus 40 (SV40). CUL7-deficient mice exhibit neonatal lethality with reduced size and vascular defects. CUL7 presumably plays a role in the DNA damage response by limiting p53 activity. CUL7 mutations have also been identified in 3-Msyndrome and the Yakuts short stature syndrome, both of which are characterized by pre- and post-natal growth retardation but with relatively normal mental and endocrine functions, suggesting that CUL7 may also be crucial for human placental development.