Anticorps Monoclonal anti-CUX1

CUX1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1B9D1

N° de cat : 68449-1-Ig

Synonymes

CASP, CCAAT displacement protein, CDP, CDP/Cut, CDP1, Clox, COY1, cut like homeobox 1, CUTL1, CUX, Cux/CDP, CUX1, GOLIM6, Homeobox protein cut like 1, Homeobox protein cux 1, Nbla10317, p100, p110, p200, p75

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Galerie de données de validation

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  WB analysis using 68449-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68449-1-Ig (CUX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules HSC-T6, cellules K-562, cellules LNCaP, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68449-1-Ig cible CUX1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CUX1 Protéine recombinante Ag28528
• Nom complet: cut-like homeobox 1
• Masse moléculaire calculée: 676 aa, 77 kDa
• Poids moléculaire observé: 80, 200 kDa
• Numéro d’acquisition GenBank: BC025422
• Symbole du gène: CUX1
• Identification du gène (NCBI): 1523
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ]. This antibody can recognizes full-length p200 Cux1 and CASP protein (70~80kDa).

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