Anticorps Recombinant de lapin anti-CUX2

• Phare
• Validé par KD/KO

CUX2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230235G1

N° de cat : 82933-1-RR

Synonymes

Homeobox protein cux-2, Homeobox protein cut-like 2, CUTL2, cut like homeobox 2, CDP2

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Galerie de données de validation

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  WB analysis using 82933-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82933-1-RR (CUX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 82933-1-RR

  WB result of CUX2 antibody (82933-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUX2 transfected SH-SY5Y cells.

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  IF Staining of HeLa using 82933-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 82933-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82933-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82933-1-RR against Human CUX2 were performed. The affinity constant is 1.02 nM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HepG2, cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82933-1-RR cible CUX2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CUX2 Protéine recombinante Ag18809
• Nom complet: cut-like homeobox 2
• Masse moléculaire calculée: 1486 aa, 162 kDa
• Poids moléculaire observé: 162 kDa
• Numéro d’acquisition GenBank: BC151245
• Symbole du gène: CUX2
• Identification du gène (NCBI): 23316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cut like homeobox 2 (CUX2) also named as, CDP2 or CUTL2, is a 1486 amino acid protein, which contains one homeobox DNA-binding domain and three CUT DNA-binding domains. CUX2 localizes in the nucleus and belongs to the CUT homeobox family. CUX2 may as a transcription factor be involved in neural specification.

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