Anticorps Recombinant de lapin anti-CX3CL1

CX3CL1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240738E2

N° de cat : 83738-5-PBS

Synonymes

Scyd1, Cxc3, CX3C, ABCD 3, 240738E2

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Galerie de données de validation

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  WB analysis using 83738-5-RR (same clone as 83738-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83738-5-RR (Cx3cl1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83738-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83738-5-RR (same clone as 83738-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83738-5-RR (CX3CL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83738-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83738-5-RR (same clone as 83738-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83738-5-RR (CX3CL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83738-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83738-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83738-5-RR against Mouse CX3CL1 were performed. The affinity constant is 0.744 nM.

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Informations sur le produit

83738-5-PBS cible CX3CL1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: chemokine (C-X3-C motif) ligand 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 55-75 kDa
• Numéro d’acquisition GenBank: NM_009142.3
• Symbole du gène: Cx3cl1
• Identification du gène (NCBI): 20312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1 (PMID: 9479488; 23125415). CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation (PMID: 21045240). CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage (PMID: 11495925; 12714508).

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