Anticorps Monoclonal anti-CX3CR1

CX3CR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

3D3B8

N° de cat : 66915-1-PBS

Synonymes

Beta chemokine receptor like 1, Beta chemokine receptor-like 1, C X3 C CKR 1, CCRL1, CMK BRL 1

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Galerie de données de validation

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  WB analysis using 66915-1-Ig (same clone as 66915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66915-1-Ig (same clone as 66915-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66915-1-Ig (same clone as 66915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66915-1-Ig (CX3CR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 66915-1-Ig (same clone as 66915-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CX3CR1 antibody (66915-1-Ig, Clone: 3D3B8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66915-1-PBS cible CX3CR1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CX3CR1 Protéine recombinante Ag27698
• Nom complet: chemokine (C-X3-C motif) receptor 1
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC028078
• Symbole du gène: CX3CR1
• Identification du gène (NCBI): 1524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CX3CR1, also known as GPR13, V28, and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed in microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as a coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

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