Anticorps Recombinant de lapin anti-CXCL1

CXCL1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240990E5

N° de cat : 83937-1-PBS

Synonymes

C X C motif chemokine 1, C-X-C motif chemokine 1, CXCL 1, GRO-alpha(4-73), GRO-alpha(5-73)

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00922-2

  Sandwich ELISA standard curve of MP00922-2, Human CXCL1 Recombinant Matched Antibody Pair - PBS only. 83937-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2917. 83937-2-PBS was biotinylated as the detection antibody. Range: 31.25-2000 pg/mL

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  Sample test of MP00922-2

  Serum of tweleve individual healthy human donors was measured. The human CXCL1 concentration of detected samples was determined to be 110.0 pg/mL with a range of 0.6 - 1,164.0 pg/mL.

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  Sample test of MP00922-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean CXCL1 concentration was determined to be 32.4 pg/mL in unstimulated PBMC supernatant, 9,728.4 pg/mL in PHA stimulated PBMC supernatant.

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  Affinity and Kinetic Characterization of 83937-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83937-1-PBS against Human CXCL1 was performed. The affinity constant is 0.278 nM.

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Informations sur le produit

83937-1-PBS cible CXCL1 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CXCL1 Protéine recombinante Ag2917
• Nom complet: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Masse moléculaire calculée: 8 kDa to 14 kDa
• Numéro d’acquisition GenBank: BC011976
• Symbole du gène: CXCL1
• Identification du gène (NCBI): 2919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

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