Anticorps Recombinant de lapin anti-CXCL10/IP-10

CXCL10/IP-10 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242013E11

N° de cat : 85307-1-RR

Synonymes

CXCL10, CXCL10/IP10, IP10, 10 kDa interferon gamma-induced protein, C X C motif chemokine 10

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Galerie de données de validation

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  WB analysis of THP-1 using 85307-1-RR

  IFN gamma, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 85307-1-RR (CXCL10/IP-10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 85307-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85307-1-RR (CXCL10/IP-10 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 85307-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85307-1-RR (CXCL10/IP-10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85307-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85307-1-RR (CXCL10/IP-10 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85307-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85307-1-RR (CXCL10/IP-10 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 85307-1-RR

  Immunofluorescent analysis of (4% PFA) fixed IFN gamma, LPS and Brefeldin A treated THP-1 cells using CXCL10/IP-10 antibody (85307-1-RR, Clone: 242013E11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: IFN gamma, LPS and Brefeldin A treated THP-1 cells,
• Résultats positifs en IHC: human ovary cancer tissue, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: IFN gamma, LPS and Brefeldin A treated THP-1 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85307-1-RR cible CXCL10/IP-10 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CXCL10/IP-10 Protéine recombinante Eg2489
• Nom complet: chemokine (C-X-C motif) ligand 10
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC010954
• Symbole du gène: CXCL10
• Identification du gène (NCBI): 3627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CXCL10 (also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.

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