Anticorps Recombinant de lapin anti-CXCL8/IL-8

CXCL8/IL-8 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240889C4

N° de cat : 83903-4-RR

Synonymes

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

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Galerie de données de validation

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  WB analysis of THP-1 using 83903-4-RR

  PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83903-4-RR (CXCL8,IL8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 83903-4-RR

  Immunofluorescent analysis of (4% PFA) fixed PMA and Brefeldin A treated THP-1 cells using CXCL8/IL-8 antibody (83903-4-RR, Clone: 240889C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83903-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83903-4-RR against Human CXCL8/IL-8 were performed. The affinity constant is 0.165 nM.

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Applications testées

• Résultats positifs en WB: PMA, LPS and Brefeldin A treated THP-1 cells,
• Résultats positifs en IF/ICC: PMA and Brefeldin A treated THP-1 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83903-4-RR cible CXCL8/IL-8 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 8
• Masse moléculaire calculée: 99 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC013615
• Symbole du gène: IL-8
• Identification du gène (NCBI): 3576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).

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