Anticorps Recombinant de lapin anti-CXCL8/IL-8

CXCL8/IL-8 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240889B2

N° de cat : 98137-1-PBS

Synonymes

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

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Galerie de données de validation

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  FC experiment of human PBMCs using 98137-1-RR (same clone as 98137-1-PBS)

  1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98137-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98137-1-RR (same clone as 98137-1-PBS)

  1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98137-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98137-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98137-1-RR against Human CXCL8/IL-8 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

98137-1-PBS cible CXCL8/IL-8 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 8
• Masse moléculaire calculée: 99 aa, 11 kDa
• Numéro d’acquisition GenBank: BC013615
• Symbole du gène: IL-8
• Identification du gène (NCBI): 3576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

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