Anticorps Monoclonal anti-CXCR3B-specific

• Phare
• Validé par KD/KO

CXCR3B-specific Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

15

CloneNo.

1B2D6

N° de cat : 60065-1-Ig

Synonymes

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3B, CXCR3B-specific, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR

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Galerie de données de validation

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  WB analysis of MOLT-4 using 60065-1-Ig

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60065-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60065-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 60065-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 60065-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 60065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 60065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 60065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of U-937 using 60065-1-Ig

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human CXCR3B-specific (60065-1-Ig, Clone:1B2D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MOLT-4, cellules HT-29, cellules Jurkat, cellules K-562, cellules U-937
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu cérébral humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules U-937,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• FC: See 3 publications below

Informations sur le produit

60065-1-Ig cible CXCR3B-specific dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: chemokine (C-X-C motif) receptor 3
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: AF469635
• Symbole du gène: CXCR3
• Identification du gène (NCBI): 2833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.

Publications

Species	Application	Title
human	WB	Arterioscler Thromb Vasc Biol; Pericyte regulation of vascular remodeling through the CXC receptor 3.; Authors - Bodnar Richard J RJ ; KD Validated
human	WB,IF	Int J Obes (Lond); Upregulation of angiostatic chemokines IP-10/CXCL10 and I-TAC/CXCL11 in human obesity and their implication for adipose tissue angiogenesis.; Authors - Luisa Hueso
human	WB, IF	Mol Cancer; Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion.; Authors - Wu Qian Q
human	FC	Cell Commun Signal; Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer.; Authors - Bo Ma
human	WB,FC	J Biol Chem; A novel CXCR3-B chemokine receptor-induced growth-inhibitory signal in cancer cells is mediated through the regulation of Bach-1 protein and Nrf2 protein nuclear translocation.; Authors - Murugabaskar Balan ; KD Validated
mouse	IF	Food Funct; Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway.; Authors - Jinhong Xu

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