Anticorps Monoclonal anti-CXCR4

• Phare
• Validé par KD/KO

CXCR4 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

39

CloneNo.

4B5E4

N° de cat : 60042-1-Ig

Synonymes

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM

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Galerie de données de validation

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  WB analysis of HeLa using 60042-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 60042-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 60042-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 60042-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60042-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 60042-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60042-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 60042-1-Ig

  1X10^6 Jurkat cells were stained with 0.2ug CXCR4 antibody (60042-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Daudi, cellules Jurkat, cellules K-562, cellules Ramos, cellules THP-1, cellules U-937
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu cérébral de souris, tissu de cancer de la prostate humain, tissu de cancer du sein humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 35 publications below
• IHC: See 8 publications below
• IF: See 9 publications below
• CoIP: See 2 publications below

Informations sur le produit

60042-1-Ig cible CXCR4 dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CXCR4 Protéine recombinante Ag1528
• Nom complet: chemokine (C-X-C motif) receptor 4
• Masse moléculaire calculée: 352 aa, 40 kDa
• Poids moléculaire observé: 60~70 kDa
• Numéro d’acquisition GenBank: BC020968
• Symbole du gène: CXCR4
• Identification du gène (NCBI): 7852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

Publications

Species	Application	Title
human	WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang
mouse	WB	Nat Commun; HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity.; Authors - Hiroaki Semba
mouse	WB,IF	J Exp Med; CXCR4 regulates Plasmodium development in mouse and human hepatocytes.; Authors - Hironori Bando ; KD Validated
human	WB	Nanoscale; Mitomycin C-treated human-induced pluripotent stem cells as a safe delivery system of gold nanorods for targeted photothermal therapy of gastric cancer; Authors - Meng Yang
human	WB	ACS Appl Mater Interfaces; Surface Functionalization of Polymeric Nanoparticles with Umbilical Cord-Derived Mesenchymal Stem Cell Membrane for Tumor-Targeted Therapy.; Authors - Na Yang
human	IF	Mol Oncol; Growth arrest-specific protein 2 (GAS2) interacts with CXCR4 to promote T-cell leukemogenesis partially via c-MYC; Authors - Yun Zhao

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