Anticorps Recombinant de lapin anti-CXCR4

CXCR4 Recombinant Antibody for IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

241734B2

N° de cat : 84904-1-RR

Synonymes

241734B2, CD184, C-X-C chemokine receptor type 4, CXC R4, CXCR 4

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Galerie de données de validation

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  IF Staining of mouse testis using 84904-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 84904-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Jurkat using 84904-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:125-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84904-1-RR cible CXCR4 dans les applications de IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: chemokine (C-X-C motif) receptor 4
• Masse moléculaire calculée: 352 aa, 40 kDa
• Numéro d’acquisition GenBank: BC020968
• Symbole du gène: CXCR4
• Identification du gène (NCBI): 7852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

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