Anticorps Recombinant de lapin anti-CXCR4/CD184

CXCR4/CD184 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241956H2

N° de cat : 98233-1-RR

Synonymes

241956H2, CD184, Cmkar4, C-X-C chemokine receptor type 4, Cxcr4

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Galerie de données de validation

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  FC experiment of mouse thymocytes using 98233-1-RR

  1x10^6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse CXCR4/CD184 Rabbit Recombinant Antibody (98233-1-RR, Clone: 241956H2) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

• 

  FC experiment of mouse thymocytes using 98233-1-RR

  1x10^6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse CXCR4/CD184 Rabbit Recombinant Antibody (98233-1-RR, Clone: 241956H2)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98233-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98233-1-RR against Mouse CXCR4/CD184 were performed. The affinity constant is 1.71 nM.

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Applications testées

• Résultats positifs en cytométrie: thymocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98233-1-RR cible CXCR4/CD184 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CXCR4/CD184 Protéine recombinante Eg2231
• Nom complet: chemokine (C-X-C motif) receptor 4
• Masse moléculaire calculée: 40kDa
• Numéro d’acquisition GenBank: NM_009911.3
• Symbole du gène: Cxcr4
• Identification du gène (NCBI): 12767
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).

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