Anticorps Polyclonal de lapin anti-CXCR5

CXCR5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28239-1-PBS

Synonymes

MDR 15, CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1

---

Galerie de données de validation

• 

  WB analysis using 28239-1-AP (same clone as 28239-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28239-1-AP (CXCR5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 28239-1-AP (same clone as 28239-1-PBS)

  WB result of CXCR5 antibody (28239-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CXCR5 transfected Jurkat cells. This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28239-1-AP (same clone as 28239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28239-1-AP (CXCR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

28239-1-PBS cible CXCR5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXCR5 Protéine recombinante Ag28271
• Nom complet: chemokine (C-X-C motif) receptor 5
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: NM_001716
• Symbole du gène: CXCR5
• Identification du gène (NCBI): 643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Initially discovered in 1992 as Burkitt's lymphoma receptor 1, CXCR5 (chemokine receptor 5, also known as CD185) is a seven-transmembrane G protein-coupled receptor (GPCR) protein (PMID: 32994482). CXCR5 is essential for naïve B-cell migration to and maturation in the lymph nodes and spleen, as well as for cluster of differentiation 4 T-cell (TFH and TCM) homing and interaction with B cells (PMID: 21471443). Moreover, CXCR5 is involved in the regulation of neural stem cells (NSC) and neuronal regeneration in the mouse brain, neuronal differentiation, neurogenesis, gliogenesis, and synaptogenesis (PMID: 37460877).

{{ptg:RelatedPrimaryAntibodies}}