Anticorps Polyclonal de lapin anti-CFAP47

CFAP47 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, CoIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 20837-1-AP

Synonymes

CXorf22, CHDC2, Cilia- and flagella-associated protein 47, CXorf30, CXorf59

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Galerie de données de validation

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  WB analysis of mouse lung using 20837-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20837-1-AP (CXorf22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse kidney using 20837-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CFAP47 antibody (20837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu pulmonaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

20837-1-AP cible CFAP47 dans les applications de WB, IHC, IF-P, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CFAP47 Protéine recombinante Ag14910
• Nom complet: chromosome X open reading frame 22
• Masse moléculaire calculée: 976 aa, 110 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC027936
• Symbole du gène: CFAP47/CXorf22
• Identification du gène (NCBI): 170063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CFAP47 (Cilia- and flagella-associated protein 47), formerly known as CXorf22, CXorf59, is located on the human chromosome X and highly expressed in the testis and encodes a cilia- and flagella-associated protein. CFAP47 was expressed in primary cilia of human kidney tubules, and knockout (KO) mice exhibited vacuolation of tubular cells and tubular dilation, providing evidence that CFAP47 is a causative gene involved in cyst formation (PMID: 33472045, 38633811). CFAP47-mutated male mice are sterile, with decreased sperm motility and abnormal flagella morphology (PMID: 37424856).

Publications

Species	Application	Title
mouse,human	WB,IF,CoIP	Mol Hum Reprod; WDR87 interacts with CFAP47 protein in the middle piece of spermatozoa flagella to participate in sperm tail assembly; Authors - Hong-Qing Liao
human	IF	medRxiv; CFAP47 is a novel causative gene implicated in X-linked polycystic kidney disease; Authors - Takayasu Mori

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