Anticorps Polyclonal de lapin anti-CYB5B

• Phare
• Validé par KD/KO

CYB5B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15469-1-AP

Synonymes

CYB5 M, CYB5B, CYB5M, CYPB5M, Cytochrome b5 type B, OMB5

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Galerie de données de validation

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  WB analysis using 15469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:4000 incubated at 4 degree celsius over night.

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  WB analysis of human liver using 15469-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15469-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 15469-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15469-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15469-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15469-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 15469-1-AP

  IP Result of anti-CYB5B (IP:15469-1-AP, 3ug; Detection:15469-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15469-1-AP (CYB5B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15469-1-AP (CYB5B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15469-1-AP (CYB5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15469-1-AP (CYB5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15469-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15469-1-AP (CYB5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu de glande surrénale humain, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

15469-1-AP cible CYB5B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYB5B Protéine recombinante Ag7771
• Nom complet: cytochrome b5 type B (outer mitochondrial membrane)
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 20-21 kDa
• Numéro d’acquisition GenBank: BC004373
• Symbole du gène: CYB5B
• Identification du gène (NCBI): 80777
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYB5B(Cytochrome b5 type B) is also named as CYB5M, OMB5 and belongs to the cytochrome b5 family. It is a 21 kDa membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. This protein is expressed on the plasma membrane of lymphoma cells but not normal lymphocytes, reactive lymphocytes or bone marrow precursor cells(PMID:20100355). The full length protein has a propeptide with 11 amino acids.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190.; Authors - Paige Dickson ; KD Validated
human	IHC	Front Mol Biosci; Comprehensive Analysis of mTORC1 Signaling Pathway-Related Genes in the Prognosis of HNSCC and the Response to Chemotherapy and Immunotherapy.; Authors - Zhao Ding
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková