Anticorps Polyclonal de lapin anti-CYP11B2

CYP11B2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 20968-1-AP

Synonymes

CYP11BL, CYP11B, CPN2, Corticosterone 18-monooxygenase, CYP11B2, Aldosterone-synthesizing enzyme

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Galerie de données de validation

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  WB analysis using 20968-1-AP

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 20968-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human adrenal gland using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human adrenal gland using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SKOV-3 using 20968-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 20968-1-AP (CYP11B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, tissu de glande surrénale de porc
• Résultats positifs en IHC: tissu de glande surrénale humain, tissu de cancer du foie humain, tissu de cirrhose hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SKOV-3

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

20968-1-AP cible CYP11B2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome P450, family 11, subfamily B, polypeptide 2
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: NM_000498
• Symbole du gène: CYP11B2
• Identification du gène (NCBI): 1585
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).

Publications

Species	Application	Title
human	IHC	Nat Commun; Adrenal tropism of SARS-CoV-2 and adrenal findings in a post-mortem case series of patients with severe fatal COVID-19.; Authors - Tanja Paul
human	WB,IHC	Cell Death Dis; Aldosterone from endometrial glands is benefit for human decidualization.; Authors - Shu-Yun Li
mouse	IF	PeerJ; Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm; Authors - Jianyi Li
human	IHC	Eur J Nucl Med Mol Imaging; Total or partial adrenalectomy for aldosterone-producing adenoma: can 68Ga-Pentixafor PET/CT predict surgical outcomes; Authors - Wen-Cai Zheng

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