Anticorps Polyclonal de lapin anti-CYP11B2

CYP11B2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20968-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 20968-1-AP (same clone as 20968-1-PBS)

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 20968-1-AP (same clone as 20968-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human adrenal gland using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human adrenal gland using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 20968-1-AP (same clone as 20968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 20968-1-AP (same clone as 20968-1-PBS)

  Immunofluorescent analysis of SKOV-3 cells using 20968-1-AP (CYP11B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20968-1-PBS cible CYP11B2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome P450, family 11, subfamily B, polypeptide 2
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: NM_000498
• Symbole du gène: CYP11B2
• Identification du gène (NCBI): 1585
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).

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