Anticorps Recombinant de lapin anti-CYP1A2-Specific

CYP1A2-Specific Recombinant Antibody for IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241922C4

N° de cat : 84751-1-PBS

Synonymes

CYP1A2, CYPIA2, CP1A2, CP12, Cholesterol 25-hydroxylase

---

Galerie de données de validation

• 

  IHC staining of mouse liver using 84751-1-RR (same clone as 84751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84751-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84751-1-RR (same clone as 84751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84751-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

84751-1-PBS cible CYP1A2-Specific dans les applications de IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome P450, family 1, subfamily A, polypeptide 2
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_000761
• Symbole du gène: CYP1A2
• Identification du gène (NCBI): 1544
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

{{ptg:RelatedPrimaryAntibodies}}