Anticorps Recombinant de lapin anti-CYP1A2-Specific

CYP1A2-Specific Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241922C4

N° de cat : 84751-1-RR

Synonymes

CYP1A2, 241922C4, Cholesterol 25-hydroxylase, CP12, CP1A2

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Galerie de données de validation

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  WB analysis of HepG2 using 84751-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 84751-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 84751-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human Hepatocellular cancer using 84751-1-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human Hepatocellular cancer using 84751-1-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 84751-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293
• Résultats positifs en IHC: Human Hepatocellular cancer tissue, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84751-1-RR cible CYP1A2-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome P450, family 1, subfamily A, polypeptide 2
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_000761
• Symbole du gène: CYP1A2
• Identification du gène (NCBI): 1544
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

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