Anticorps Monoclonal anti-CYP20A1

CYP20A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A9E2

N° de cat : 67433-1-PBS

Synonymes

2A9E2, CYP M, Cytochrome P450 20A1, EC:1.14.-.-, UNQ667/PRO1301

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Galerie de données de validation

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  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67433-1-PBS cible CYP20A1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CYP20A1 Protéine recombinante Ag9715
• Nom complet: cytochrome P450, family 20, subfamily A, polypeptide 1
• Masse moléculaire calculée: 462 aa, 52 kDa
• Poids moléculaire observé: 49-52 kDa
• Numéro d’acquisition GenBank: BC020616
• Symbole du gène: CYP20A1
• Identification du gène (NCBI): 57404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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