Anticorps Polyclonal de lapin anti-CYP27A1

CYP27A1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14739-1-PBS

Synonymes

Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CTX, CP27

---

Galerie de données de validation

• 

  WB analysis of mouse liver using 14739-1-AP (same clone as 14739-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 14739-1-AP (same clone as 14739-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 14739-1-AP (same clone as 14739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 14739-1-AP (same clone as 14739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14739-1-PBS cible CYP27A1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP27A1 Protéine recombinante Ag6408
• Nom complet: cytochrome P450, family 27, subfamily A, polypeptide 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC051851
• Symbole du gène: CYP27A1
• Identification du gène (NCBI): 1593
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.

{{ptg:RelatedPrimaryAntibodies}}