Anticorps Polyclonal de lapin anti-CYP2E1-Specific

• Phare
• Validé par KD/KO

CYP2E1-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

87

Avis (2)

N° de cat : 19937-1-AP

Synonymes

4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP-2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E

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Galerie de données de validation

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  WB analysis using 19937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19937-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 19937-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19937-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 19937-1-AP

  Immunofluorescent analysis of Hela cells, using CYP2E1 antibody 19937-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules Jurkat, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 80 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Informations sur le produit

19937-1-AP cible CYP2E1-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome P450, family 2, subfamily E, polypeptide 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 50-57 kDa
• Numéro d’acquisition GenBank: NM_000773
• Symbole du gène: CYP2E1
• Identification du gène (NCBI): 1571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

Publications

Species	Application	Title
mouse	WB	Cell Host Microbe; Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice; Authors - Yunong Zeng
mouse	WB	Cell Metab; Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.; Authors - Jing Yuan
mouse	WB	Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun
mouse	WB	Cell Biol Toxicol; Protecting mitochondria via inhibiting VDAC1 oligomerization alleviates ferroptosis in acetaminophen-induced acute liver injury.; Authors - Baolin Niu
mouse	WB	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with Cyp2e1 antibody at dilution of 1:5000 incubated at 4 degree overnight. Bands appeared at about 55 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse liver tissue

FH

James (Verified Customer) (11-18-2022)

Detection of CYP2E1 in liver protein extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

CYP2E1-Specific Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:19937-1-AP)