Anticorps Recombinant de lapin anti-CYP2R1

CYP2R1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241072F11

N° de cat : 84378-5-PBS

Synonymes

241072F11, EC:1.14.14.24, Vitamin D 25 hydroxylase, Vitamin D 25-hydroxylase

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Galerie de données de validation

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  WB analysis using 84378-5-RR (same clone as 84378-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84378-5-RR (CYP2R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84378-5-PBS in a different storage buffer formulation.

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  FC experiment of Hela using 84378-5-RR (same clone as 84378-5-PBS)

  1x10^6 Hela cells were intracellularly stained with 0.25 ug CYP2R1 Recombinant antibody (84378-5-RR, Clone:241072F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84378-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01251-2

  Cytometric bead array standard curve of MP01251-2, CYP2R1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84378-3-PBS. Detection antibody: 84378-5-PBS. Standard: Ag12873. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84378-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84378-5-RR against Human CYP2R1 were performed. The affinity constant is 0.243 nM.

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Informations sur le produit

84378-5-PBS cible CYP2R1 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CYP2R1 Protéine recombinante Ag12873
• Nom complet: cytochrome P450, family 2, subfamily R, polypeptide 1
• Masse moléculaire calculée: 501 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC104909
• Symbole du gène: CYP2R1
• Identification du gène (NCBI): 120227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. CYP2R1 primarily functions as a 25-hydroxylase of vitamin D, responsible for converting vitamin D to 25-hydroxyvitamin D (25-OH-D), affecting the production of the active hormone 1,25(OH)2D (PMID: 12867411). CYP2R1 enzyme is a key vitamin D 25-hydroxylase, mutations in CYP2R1 are associated with Rickets vitamin D-dependent 1B(PMID: 25942481, 15128933).

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