Anticorps Monoclonal anti-CYP3A4

CYP3A4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F11C4

N° de cat : 67110-1-PBS

Synonymes

2F11C4, Cholesterol 25-hydroxylase, CP33, CP34, CYP3A

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Galerie de données de validation

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  WB analysis using 67110-1-Ig (same clone as 67110-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67110-1-Ig (same clone as 67110-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  WB analysis of pig gall bladder using 67110-1-Ig (same clone as 67110-1-PBS)

  pig gall bladder tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 67110-1-Ig (same clone as 67110-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50718-4

  Cytometric bead array standard curve of MP50718-4, CYP3A4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67110-6-PBS. Detection antibody: 67110-1-PBS. Standard:Ag13074. Range: 0.781-100 ng/mL.

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Informations sur le produit

67110-1-PBS cible CYP3A4 dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CYP3A4 Protéine recombinante Ag13074
• Nom complet: cytochrome P450, family 3, subfamily A, polypeptide 4
• Masse moléculaire calculée: 503 aa, 57 kDa
• Poids moléculaire observé: 51-57 kDa
• Numéro d’acquisition GenBank: BC101631
• Symbole du gène: CYP3A4
• Identification du gène (NCBI): 1576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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