Anticorps Polyclonal de lapin anti-CYP46A1

CYP46A1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 12486-1-AP

Synonymes

CP46, Cholesterol 24S-hydroxylase, Cholesterol 24-monooxygenase, Cholesterol 24-hydroxylase, Cholesterol 24 hydroxylase

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Galerie de données de validation

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  WB analysis using 12486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12486-1-AP (CYP46A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 12486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12486-1-AP (CYP46A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12486-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12486-1-AP (CYP46A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12486-1-AP

  IP result of anti-CYP46A1 (IP:12486-1-AP, 4ug; Detection:12486-1-AP 1:500) with mouse brain tissue lysate 3200ug.

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  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 12486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 12486-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CYP46A1 antibody (12486-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

12486-1-AP cible CYP46A1 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP46A1 Protéine recombinante Ag3116
• Nom complet: cytochrome P450, family 46, subfamily A, polypeptide 1
• Masse moléculaire calculée: 500 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC022539
• Symbole du gène: CYP46A1
• Identification du gène (NCBI): 10858
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)

Publications

Species	Application	Title
human,mouse	WB,IHC	Cell Rep Med; Cholesterol 24-hydroxylase at the choroid plexus contributes to brain immune homeostasis; Authors - Afroditi Tsitsou-Kampeli
mouse	IF	Nat Commun; ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.; Authors - Yuanyuan Zhao
mouse	WB	EMBO Rep; NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation.; Authors - Daniel N Mitroi
mouse	WB	Oncogene; Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma.; Authors - Risheng Yang
human	WB	Neurobiol Aging; H63D mutation in hemochromatosis alters cholesterol metabolism and induces memory impairment.; Authors - Fatima Ali-Rahmani
rat	IF	Sci Rep; 24(S)-Hydroxycholesterol protects the ex vivo rat retina from injury by elevated hydrostatic pressure.; Authors - Makoto Ishikawa

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