Anticorps Monoclonal anti-CYP46A1

CYP46A1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E10D3

N° de cat : 68576-1-PBS

Synonymes

CH24H, Cholesterol 24 hydroxylase, CP46, CYP46, CYP46A1, Cytochrome P450 46A1

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Galerie de données de validation

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  WB analysis using 68576-1-Ig (same clone as 68576-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68576-1-Ig (CYP46A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68576-1-Ig (same clone as 68576-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68576-1-Ig (same clone as 68576-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68576-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68576-1-PBS cible CYP46A1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CYP46A1 Protéine recombinante Ag16786
• Nom complet: cytochrome P450, family 46, subfamily A, polypeptide 1
• Masse moléculaire calculée: 500 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC022539
• Symbole du gène: CYP46A1
• Identification du gène (NCBI): 10858
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)

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