Anticorps Polyclonal de lapin anti-CYP4A11

CYP4A11 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11688-1-AP

Synonymes

20 HETE synthase, CP4Y, CYP4A11, CYP4A2, CYP4AII, CYPIVA11, Cytochrome P 450HK omega, Cytochrome P450 4A11, Cytochrome P450HL omega, Fatty acid omega hydroxylase, Lauric acid omega hydroxylase

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Galerie de données de validation

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  WB analysis of HEK-293 using 11688-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 11688-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11688-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11688-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11688-1-AP (CYP4A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11688-1-AP (CYP4A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:300-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

11688-1-AP cible CYP4A11 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP4A11 Protéine recombinante Ag2300
• Nom complet: cytochrome P450, family 4, subfamily A, polypeptide 11
• Masse moléculaire calculée: 519 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC022851
• Symbole du gène: CYP4A11
• Identification du gène (NCBI): 1579
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
rat	WB	Food Funct; Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
rat	WB	Food Funct; Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
human	WB	Forensic Sci Int; Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia.; Authors - Liujun Han
mouse,rat	WB	Chem Biol Interact; Activation of 20-HETE/PPARs involved in reno-therapeutic effect of naringenin on diabetic nephropathy.; Authors - Shumei Ding