Anticorps Recombinant de lapin anti-CYP4F2-Specific

CYP4F2-Specific Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242307B10

N° de cat : 85320-1-RR

Synonymes

CYP4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, 242307B10, Arachidonic acid omega-hydroxylase

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Galerie de données de validation

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  WB analysis using 85320-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85320-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 85320-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 85320-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Hepatocellular Cancer using 85320-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Hepatocellular Cancer using 85320-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HuH-7, cellules L02, cellules SMMC-7721, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, human hepatocellular cancer; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85320-1-RR cible CYP4F2-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome P450, family 4, subfamily F, polypeptide 2
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 50-53 kDa
• Numéro d’acquisition GenBank: NM_001082
• Symbole du gène: CYP4F2
• Identification du gène (NCBI): 8529
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548).

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