Anticorps Polyclonal de lapin anti-CYP7A1

CYP7A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18054-1-PBS

Synonymes

Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase

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Galerie de données de validation

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  WB analysis using 18054-1-AP (same clone as 18054-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18054-1-AP (CYP7A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18054-1-AP (same clone as 18054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18054-1-AP (same clone as 18054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18054-1-AP (same clone as 18054-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7A1 antibody (18054-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18054-1-PBS cible CYP7A1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP7A1 Protéine recombinante Ag12572
• Nom complet: cytochrome P450, family 7, subfamily A, polypeptide 1
• Masse moléculaire calculée: 504 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC101777
• Symbole du gène: CYP7A1
• Identification du gène (NCBI): 1581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.

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