Anticorps Polyclonal de lapin anti-CYP7B1

CYP7B1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24889-1-PBS

Synonymes

Cytochrome P450 7B1, CP7B, CBAS3, 3-hydroxysteroid 7-alpha hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase

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Galerie de données de validation

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  WB analysis of A431 using 24889-1-AP (same clone as 24889-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24889-1-PBS cible CYP7B1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP7B1 Protéine recombinante Ag19374
• Nom complet: cytochrome P450, family 7, subfamily B, polypeptide 1
• Masse moléculaire calculée: 506 aa, 58 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC136574
• Symbole du gène: CYP7B1
• Identification du gène (NCBI): 9420
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

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