Anticorps Recombinant de lapin anti-CYP7B1

CYP7B1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230160H5

N° de cat : 82927-1-PBS

Synonymes

CP7B, CBAS3, 3-hydroxysteroid 7-alpha hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 24-hydroxycholesterol 7-alpha-hydroxylase

---

Galerie de données de validation

• 

  WB analysis using 82927-1-RR (same clone as 82927-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82927-1-RR (CYP7B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82927-1-RR (same clone as 82927-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 82927-1-RR (same clone as 82927-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82927-1-RR (same clone as 82927-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYP7B1 Recombinant antibody (82927-1-RR, Clone:230160H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

82927-1-PBS cible CYP7B1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CYP7B1 Protéine recombinante Ag19374
• Nom complet: cytochrome P450, family 7, subfamily B, polypeptide 1
• Masse moléculaire calculée: 506 aa, 58 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC136574
• Symbole du gène: CYP7B1
• Identification du gène (NCBI): 9420
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

{{ptg:RelatedPrimaryAntibodies}}