Anticorps Polyclonal de lapin anti-CYTSA

CYTSA Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25390-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 25390-1-AP (same clone as 25390-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 25390-1-AP (same clone as 25390-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 25390-1-AP (same clone as 25390-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  IP result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25390-1-PBS cible CYTSA dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYTSA Protéine recombinante Ag21896
• Nom complet: cytospin A
• Masse moléculaire calculée: 1117 aa, 125 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC136471
• Symbole du gène: CYTSA
• Identification du gène (NCBI): 23384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.

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