Anticorps Polyclonal de lapin anti-CaMKII Gamma

• Phare
• Validé par KD/KO

CaMKII Gamma Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12666-2-PBS

Synonymes

CAMK2G, CaMK-II subunit gamma, CAMK-II, CAMK, CaM kinase II subunit gamma

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Galerie de données de validation

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  WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12666-2-AP (same clone as 12666-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

  IP result of anti-CaMKII Gamma (IP:12666-2-AP, 4ug; Detection:12666-2-AP 1:1000) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12666-2-AP (CaMKII Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12666-2-AP (same clone as 12666-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12666-2-AP (CaMKII gamma antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12666-2-AP (same clone as 12666-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CaMKII Gamma antibody (12666-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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Informations sur le produit

12666-2-PBS cible CaMKII Gamma dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CaMKII Gamma Protéine recombinante Ag3348
• Nom complet: calcium/calmodulin-dependent protein kinase II gamma
• Masse moléculaire calculée: 527 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC034044
• Symbole du gène: CAMK2G
• Identification du gène (NCBI): 818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.

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