Anticorps Monoclonal anti-Cadherin-17

Cadherin-17 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B10A9

N° de cat : 60351-1-PBS

Synonymes

CDH17, 3B10A9, Cadherin 17, CDH16, HPT 1

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Galerie de données de validation

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  WB analysis of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

  Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 60351-1-Ig (same clone as 60351-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60351-1-Ig (same clone as 60351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60351-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60351-1-PBS cible Cadherin-17 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cadherin-17 Protéine recombinante Ag19488
• Nom complet: cadherin 17, LI cadherin (liver-intestine)
• Masse moléculaire calculée: 832 aa, 92 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC113464
• Symbole du gène: Cadherin-17
• Identification du gène (NCBI): 1015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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